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Cell Biolabs Inc aav dj helper free packaging system
Aav Dj Helper Free Packaging System, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav-dj+helper+free+packaging+system/aav+dj+free+helper+packaging+system/bio_rxiv__64898__2026__01__14__699532-118-10-15
Average 86 stars, based on 1 article reviews
aav dj helper free packaging system - by Bioz Stars, 2026-09
86/100 stars

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Recombinant:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Generated:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Transfection:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Bioprocessing:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Expressing:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

MANN-WHITNEY:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Construct:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Injection:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

shRNA:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

In Vivo:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Dominant Negative Mutation:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Negative Control:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice

Staining:

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAV-hSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSyn-Synaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHR-casp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Article Snippet: DJ-type AAV vectors were prepared using AAV-DJ Helper Free Packaging System (Cell Biolabs).

Article Title: A shift in the mechanisms controlling hippocampal engram formation during brain maturation
Article Snippet: AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol.

Article Title: Hippocampal sharp wave ripples underlie stress susceptibility in male mice
Article Snippet: Recombinant adeno-associated viruses (AAVs) were generated by triple transfection of the 293 AAV cell line (AAV-100; Cell Biolabs, Inc., San Diego, CA) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (VPK-400-DJ; Cell Biolabs, Inc.) and pAAV-U6-shRNA-CMV-mCherry using PEI-Max (24765; Polysciences, Inc., Warrington, PA).

Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
Article Snippet: Recombinant AAVs were generated by triple transfection of the 293 AAV cell line (Cell Biolabs, #AAV-100) with AAVdj rep-cap, pHelper from the AAV-DJ Helper Free Packaging System (Cell Biolabs, #VPK-400-DJ) and pAAV-hSyn-hM3Dq-T2A-H2B-GFP or pAAVhSyn-hM4Di-T2A-H2B-GFP or pAAV-hSyn-Mito-DsRed or pAAV-hSynSynaptophysin-mCherry or pAAV-hSyn-Synaptophysin-mSCAT3 or Nature Communications | (2025) 16:918 12 pAAV-hSyn-membrane-iRFP or pAAV-hSyn-opto-casp9_AtCRY2-PHRcasp9 or pAAV-hSyn-EGFP using PEI-Max (Polysciences, #24765).

Article Title: Protocol to visualize three distinct neuronal ensembles encoding different events in the mouse brain using genetic and viral approaches.
Article Snippet: Lesuis et al., STAR Protocols 6, 103747 June 20, 2025 a 2025 The Author(s).. Published by Elsevier Inc. Activity tagging of neuronal ensembles has become an important tool in neuroscience.. Here, we present a protocol for visualizing separate neuronal ensembles active during three distinct phases of a memory in transgenic mice.

Article Title: Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.
Article Snippet: Article Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice



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